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该测定依赖于抗原-抗体相互作用的原理，并利用酶和比色检测来量化目标分子，主要用于检测和量化生物样品中特定蛋白质、肽、抗体或抗原的存在。 ELISA测定通常应用于各个领域，包括临床诊断、药物研究和生命科学基础研究。 鉴于抗体试剂的特异性以及检测的简易性，ELISA 通常被视为生物样品定量检测的金标准。 在生物医学/生物化学领域，使用抗体-抗原结合连同信号放大的 ELISA 原理的检测非常普遍。 在研究实验室，这些检测可用来定量蛋白水平或通路激活。 酶联免疫吸附试验 (以下简称ELISA) ：是酶免疫测定技术中应用最广的技术。 其基本方法是将已知的抗原或抗体吸附在固相载体 ( 聚苯乙烯微量反应板 ) 表面，使酶标记的抗原抗体反应在固相表面进行，用洗涤法将液相中的游离成分洗除。
根據待測樣品與鍵結機制的不同，ELISA可設計出各種不同類型的檢測方式，主要以三明治法（sandwich）、間接法（indirect）、以及競爭法（competitive）三種為主。 In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface. Learn about the different methods for performing an elisa assay for protein quantitation, including assay design strategies and reagents.
什么是 ELISA（酶联免疫吸附检测）？ ELISA（酶联免疫吸附检测）是一种平板检测技术，旨在检测和定量肽、蛋白质、抗体和激素等物质。 其他名称，比如酶免疫测定（EIA）也用于描述该的技术。 在 ELISA 中，抗原必须固定化到固体表面，然后与连接到酶的抗体.
本文详细介绍了ELISA的原理、分类方法、操作步骤、结果判断及应用领域，旨在为研究人员提供一份全面的ELISA技术指南。 酶联免疫吸附实验 （Enzyme linked immunosorbent assay，ELISA）是将 抗原 或 抗体 结合在 固相载体 表面，利用抗原抗体的特异性结合以及抗体或者抗原上标记的酶催化特定底物发生 显色反应，实现目标物检测的免疫分析方法，可测至皮摩尔（pmol）级别。 在 ELISA 中，将具有特定结合特性的液体样品添加到反应室或微孔板内的固定固相上。 然后，依次孵育不同的液体试剂，从而在最终液体中产生一些光学变化 (例如，通过酶促反应产物的颜色变化)。 Elisa has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries
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