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小胶质细胞（Microglia, MG）是一种分布于大脑和脊髓的神经胶质细胞，占脑内所有细胞的10-15%【1】。 作为脑内主要的神经免疫细胞MG网络横跨整个中枢神经系统。 MG对维持大脑正常功能至关重要，具有以下三个基本功能： 1. 参与持续感知环境变化的哨兵功能（sentinel function）； 2. 促进神经元健康和正常运行的管家功能（defense function）； 3. 以及响应这些变化和提供神经保护所需的防御功能（defense function）。 MG的形态与其功能密切相关。 正常情况下静息状态的MG胞体较小、呈高度分枝状，均匀分布于整个脑实质。 小胶质细胞的形态学改变反映小胶质细胞的活化状态，而小胶质细胞的活化状态与脑内受损部位的严重程度密切相关。 小胶质细胞可以作为大脑中不同细胞功能和功能障碍的评价指标，对于小胶质细胞形态学的定量显得尤为重要。 小胶质细胞可以作为大脑中不同细胞功能和功能障碍的评价指标，对于小胶质细胞形态学的定量显得尤为重要。 下面给大家分享如何使用ImageJ对小胶质细胞形态学进行分析。
【ImageJ 2】细胞形态学分析：以小胶质细胞骨架分析为例_哔哩哔哩_bilibili 以小胶质细胞骨架分析为例进行讲解，贴合文章发表需求。 根据平台原则，插件下载链接无法提供，大家认真看视频就可以找到如何下载 图文总结：11:18 我们应用了五种最常用的基于 ImageJ 的方法来量化小胶质细胞对相同显微照片和从这些显微照片中分离出的单个小胶质细胞的刺激的形态学反应，从而可以直接比较使用这些方法生成的结果。
选择单个细胞进行详细分析，确保每组样本条件一致。 将图像转换为二值图像，保持阈值的一致性，以获得清晰的细胞形态。
为了克服这些挑战并捕捉各种生理和病理状态下小胶质细胞形态的连续性，我们描述了一种简单且可重复的自定义方法，用于小鼠脑切片中的 3D 小胶质细胞形态分析。 一文解决细胞形态分析（原视频为小胶质细胞，本文例图为神经元，图片来源网络） 0：打开图片及预处理图片将RGB转为8-bit ImageJ→Type→8-bit 黑白图 I: Skeleton analysisImageJ→Lookup Tables→Grays Process→… 为了量化小胶质细胞形态，需要用一种对细胞突起和细胞体都特异且敏感的染色剂对小胶质细胞进行标记，且该染色剂在所有表型中均能表达（通常为Iba-1）。
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